Construct: shRNA TRCN0000332661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007559.4-481s21c1
DNA Barcode:
GCTGACCTGATTATGAGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp8b (12164)
Gene Description:
bone morphogenetic protein 8b
Transcript:
RefSeq NM_007559.4 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACCTGATTATGAGCTTT
Hairpin Sequence:
5'-CCGG-GCTGACCTGATTATGAGCTTT-CTCGAG-AAAGCTCATAATCAGGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACCTGATTATGAGCTTTCTCGAGAAAGCTCATAATCAGGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACCTGATTATGAGCTTTCTCGAGAAAGCTCATAATCAGGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000098051