Construct: shRNA TRCN0000332754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011965.2-208s21c1
DNA Barcode:
GTTCAGCAACAGTTGGTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psma1 (26440)
Gene Description:
proteasome (prosome, macropain) subunit, alpha type 1
Transcript:
RefSeq NM_011965.2 (CURRENT)
Match location:
Position 208 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAGCAACAGTTGGTCTAA
Hairpin Sequence:
5'-CCGG-GTTCAGCAACAGTTGGTCTAA-CTCGAG-TTAGACCAACTGTTGCTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAGCAACAGTTGGTCTAACTCGAGTTAGACCAACTGTTGCTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAGCAACAGTTGGTCTAACTCGAGTTAGACCAACTGTTGCTGAAC-3'

Other clones with same target sequence:

  1. TRCN0000032192