Construct: shRNA TRCN0000332828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008231.4-713s21c1
DNA Barcode:
GAAGGGAAACTGGTGATCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hdgf (15191)
Gene Description:
hepatoma-derived growth factor
Transcript:
RefSeq NM_008231.4 (CURRENT)
Match location:
Position 713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGGAAACTGGTGATCGAT
Hairpin Sequence:
5'-CCGG-GAAGGGAAACTGGTGATCGAT-CTCGAG-ATCGATCACCAGTTTCCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGGAAACTGGTGATCGATCTCGAGATCGATCACCAGTTTCCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGGAAACTGGTGATCGATCTCGAGATCGATCACCAGTTTCCCTTC-3'

Other clones with same target sequence:

  1. TRCN0000089226