Construct: shRNA TRCN0000332954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020761.2-4768s21c1
DNA Barcode:
CGACTACTACATCTCCGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPTOR (57521)
Gene Description:
regulatory associated protein of MTOR complex 1
Transcript:
RefSeq NM_020761.2 (NON-CURRENT)
Match location:
Position 4768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTACTACATCTCCGTGTA
Hairpin Sequence:
5'-CCGG-CGACTACTACATCTCCGTGTA-CTCGAG-TACACGGAGATGTAGTAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTACTACATCTCCGTGTACTCGAGTACACGGAGATGTAGTAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTACTACATCTCCGTGTACTCGAGTACACGGAGATGTAGTAGTCG-3'

Other clones with same target sequence:

  1. TRCN0000221530