Construct: shRNA TRCN0000332987

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031407.4-9529s21c1
DNA Barcode:
CGACGAGAACTAGCACAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HUWE1 (10075)
Gene Description:
HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_031407.4 (NON-CURRENT)
Match location:
Position 9529 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332987)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGAGAACTAGCACAGAAT
Hairpin Sequence:
5'-CCGG-CGACGAGAACTAGCACAGAAT-CTCGAG-ATTCTGTGCTAGTTCTCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGAGAACTAGCACAGAATCTCGAGATTCTGTGCTAGTTCTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGAGAACTAGCACAGAATCTCGAGATTCTGTGCTAGTTCTCGTCG-3'

Other clones with same target sequence:

  1. TRCN0000073306