Construct: shRNA TRCN0000333022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015246.2-3023s21c1
DNA Barcode:
CCCTTGCAGTTCTCTCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGRN1 (23295)
Gene Description:
mahogunin ring finger 1
Transcript:
RefSeq NM_015246.2 (NON-CURRENT)
Match location:
Position 3023 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGCAGTTCTCTCTGATT
Hairpin Sequence:
5'-CCGG-CCCTTGCAGTTCTCTCTGATT-CTCGAG-AATCAGAGAGAACTGCAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGCAGTTCTCTCTGATTCTCGAGAATCAGAGAGAACTGCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGCAGTTCTCTCTGATTCTCGAGAATCAGAGAGAACTGCAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000033789