Construct: shRNA TRCN0000333050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005956.3-1259s21c1
DNA Barcode:
CCAAGCGTTTCCTGGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTHFD1 (4522)
Gene Description:
methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
Transcript:
RefSeq NM_005956.3 (NON-CURRENT)
Match location:
Position 1259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCGTTTCCTGGAGAAAT
Hairpin Sequence:
5'-CCGG-CCAAGCGTTTCCTGGAGAAAT-CTCGAG-ATTTCTCCAGGAAACGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCGTTTCCTGGAGAAATCTCGAGATTTCTCCAGGAAACGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCGTTTCCTGGAGAAATCTCGAGATTTCTCCAGGAAACGCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000036527