Construct: shRNA TRCN0000333061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004068.3-313s21c1
DNA Barcode:
CACCAGCTTCTTCCACGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP2M1 (1173)
Gene Description:
adaptor related protein complex 2 subunit mu 1
Transcript:
RefSeq NM_004068.3 (NON-CURRENT)
Match location:
Position 313 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGCTTCTTCCACGTTAA
Hairpin Sequence:
5'-CCGG-CACCAGCTTCTTCCACGTTAA-CTCGAG-TTAACGTGGAAGAAGCTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGCTTCTTCCACGTTAACTCGAGTTAACGTGGAAGAAGCTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGCTTCTTCCACGTTAACTCGAGTTAACGTGGAAGAAGCTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000060239