Construct: shRNA TRCN0000333069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022126.2-368s21c1
DNA Barcode:
CAACCCAAACTGTGTGGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LHPP (64077)
Gene Description:
phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Transcript:
RefSeq NM_022126.2 (NON-CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCCAAACTGTGTGGTAAT
Hairpin Sequence:
5'-CCGG-CAACCCAAACTGTGTGGTAAT-CTCGAG-ATTACCACACAGTTTGGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCCAAACTGTGTGGTAATCTCGAGATTACCACACAGTTTGGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCCAAACTGTGTGGTAATCTCGAGATTACCACACAGTTTGGGTTG-3'

Other clones with same target sequence:

  1. TRCN0000049993