Construct: shRNA TRCN0000333165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005804.2-943s21c1
DNA Barcode:
GCTGGAGTTTAACCAGGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX39A (10212)
Gene Description:
DExD-box helicase 39A
Transcript:
RefSeq NM_005804.2 (NON-CURRENT)
Match location:
Position 943 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGTTTAACCAGGTGAT
Hairpin Sequence:
5'-CCGG-GCTGGAGTTTAACCAGGTGAT-CTCGAG-ATCACCTGGTTAAACTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGTTTAACCAGGTGATCTCGAGATCACCTGGTTAAACTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGTTTAACCAGGTGATCTCGAGATCACCTGGTTAAACTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000050669