Construct: shRNA TRCN0000333258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030810.2-1309s21c1
DNA Barcode:
GCCAAGCGAAAGACGAACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TXNDC5 (81567)
Gene Description:
thioredoxin domain containing 5
Transcript:
RefSeq NM_030810.2 (NON-CURRENT)
Match location:
Position 1309 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGCGAAAGACGAACTTT
Hairpin Sequence:
5'-CCGG-GCCAAGCGAAAGACGAACTTT-CTCGAG-AAAGTTCGTCTTTCGCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGCGAAAGACGAACTTTCTCGAGAAAGTTCGTCTTTCGCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGCGAAAGACGAACTTTCTCGAGAAAGTTCGTCTTTCGCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000064354