Construct: shRNA TRCN0000333324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002495.2-133s21c1
DNA Barcode:
GAGGACTTCCACATGGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFS4 (4724)
Gene Description:
NADH:ubiquinone oxidoreductase subunit S4
Transcript:
RefSeq NM_002495.2 (NON-CURRENT)
Match location:
Position 133 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACTTCCACATGGAGATT
Hairpin Sequence:
5'-CCGG-GAGGACTTCCACATGGAGATT-CTCGAG-AATCTCCATGTGGAAGTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACTTCCACATGGAGATTCTCGAGAATCTCCATGTGGAAGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACTTCCACATGGAGATTCTCGAGAATCTCCATGTGGAAGTCCTC-3'

Other clones with same target sequence:

  1. TRCN0000036632