Construct: shRNA TRCN0000333326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005176.5-398s21c1
DNA Barcode:
CGACCGGAGATACTGACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5MC2 (517)
Gene Description:
ATP synthase membrane subunit c locus 2
Transcript:
RefSeq NM_005176.5 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCGGAGATACTGACAGAT
Hairpin Sequence:
5'-CCGG-CGACCGGAGATACTGACAGAT-CTCGAG-ATCTGTCAGTATCTCCGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCGGAGATACTGACAGATCTCGAGATCTGTCAGTATCTCCGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCGGAGATACTGACAGATCTCGAGATCTGTCAGTATCTCCGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000043490