Construct: shRNA TRCN0000333376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015191.1-827s21c1
DNA Barcode:
CCGTATTTCATGTCAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIK2 (23235)
Gene Description:
salt inducible kinase 2
Transcript:
RefSeq NM_015191.1 (NON-CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATTTCATGTCAGAAGAT
Hairpin Sequence:
5'-CCGG-CCGTATTTCATGTCAGAAGAT-CTCGAG-ATCTTCTGACATGAAATACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATTTCATGTCAGAAGATCTCGAGATCTTCTGACATGAAATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATTTCATGTCAGAAGATCTCGAGATCTTCTGACATGAAATACGG-3'

Other clones with same target sequence:

  1. TRCN0000037497