Construct: shRNA TRCN0000333400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006384.3-600s21c1
DNA Barcode:
GTCTGAGATGAAGCAGCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIB1 (10519)
Gene Description:
calcium and integrin binding 1
Transcript:
RefSeq NM_006384.3 (NON-CURRENT)
Match location:
Position 600 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGAGATGAAGCAGCTCAT
Hairpin Sequence:
5'-CCGG-GTCTGAGATGAAGCAGCTCAT-CTCGAG-ATGAGCTGCTTCATCTCAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGAGATGAAGCAGCTCATCTCGAGATGAGCTGCTTCATCTCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGAGATGAAGCAGCTCATCTCGAGATGAGCTGCTTCATCTCAGAC-3'

Other clones with same target sequence:

  1. TRCN0000053590