Construct: shRNA TRCN0000333416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000532.3-580s21c1
DNA Barcode:
GCAGACATCTTTCTGAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCCB (5096)
Gene Description:
propionyl-CoA carboxylase subunit beta
Transcript:
RefSeq NM_000532.3 (NON-CURRENT)
Match location:
Position 580 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACATCTTTCTGAGGAAT
Hairpin Sequence:
5'-CCGG-GCAGACATCTTTCTGAGGAAT-CTCGAG-ATTCCTCAGAAAGATGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACATCTTTCTGAGGAATCTCGAGATTCCTCAGAAAGATGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACATCTTTCTGAGGAATCTCGAGATTCCTCAGAAAGATGTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000078432