Construct: shRNA TRCN0000333437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004691.4-218s21c1
DNA Barcode:
GAGACGCTAGAGGACTTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0D1 (9114)
Gene Description:
ATPase H+ transporting V0 subunit d1
Transcript:
RefSeq NM_004691.4 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACGCTAGAGGACTTGAAA
Hairpin Sequence:
5'-CCGG-GAGACGCTAGAGGACTTGAAA-CTCGAG-TTTCAAGTCCTCTAGCGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACGCTAGAGGACTTGAAACTCGAGTTTCAAGTCCTCTAGCGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACGCTAGAGGACTTGAAACTCGAGTTTCAAGTCCTCTAGCGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000038558