Construct: shRNA TRCN0000333448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009.3-282s21c1
DNA Barcode:
GCGCCTCACTAACTCCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS5 (6193)
Gene Description:
ribosomal protein S5
Transcript:
RefSeq NM_001009.3 (NON-CURRENT)
Match location:
Position 282 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTCACTAACTCCATGAT
Hairpin Sequence:
5'-CCGG-GCGCCTCACTAACTCCATGAT-CTCGAG-ATCATGGAGTTAGTGAGGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTCACTAACTCCATGATCTCGAGATCATGGAGTTAGTGAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTCACTAACTCCATGATCTCGAGATCATGGAGTTAGTGAGGCGC-3'

Other clones with same target sequence:

  1. TRCN0000074757