Construct: shRNA TRCN0000333465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001323.1-862s21c1
DNA Barcode:
CGGCATACTTATTCAAGGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP2B1 (490)
Gene Description:
ATPase plasma membrane Ca2+ transporting 1
Transcript:
RefSeq NM_001001323.1 (NON-CURRENT)
Match location:
Position 862 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATACTTATTCAAGGCAA
Hairpin Sequence:
5'-CCGG-CGGCATACTTATTCAAGGCAA-CTCGAG-TTGCCTTGAATAAGTATGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATACTTATTCAAGGCAACTCGAGTTGCCTTGAATAAGTATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATACTTATTCAAGGCAACTCGAGTTGCCTTGAATAAGTATGCCG-3'

Other clones with same target sequence:

  1. TRCN0000043072