Construct: shRNA TRCN0000333542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002402.2-1392s21c1
DNA Barcode:
GCTCTGACTAAGGTTGACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEST (4232)
Gene Description:
mesoderm specific transcript
Transcript:
RefSeq NM_002402.2 (NON-CURRENT)
Match location:
Position 1392 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGACTAAGGTTGACATA
Hairpin Sequence:
5'-CCGG-GCTCTGACTAAGGTTGACATA-CTCGAG-TATGTCAACCTTAGTCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGACTAAGGTTGACATACTCGAGTATGTCAACCTTAGTCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGACTAAGGTTGACATACTCGAGTATGTCAACCTTAGTCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000075318