Construct: shRNA TRCN0000333550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006185.2-1297s21c1
DNA Barcode:
CCTTGAAGAGAAGAACGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUMA1 (4926)
Gene Description:
nuclear mitotic apparatus protein 1
Transcript:
RefSeq NM_006185.2 (NON-CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGAAGAGAAGAACGAAAT
Hairpin Sequence:
5'-CCGG-CCTTGAAGAGAAGAACGAAAT-CTCGAG-ATTTCGTTCTTCTCTTCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGAAGAGAAGAACGAAATCTCGAGATTTCGTTCTTCTCTTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGAAGAGAAGAACGAAATCTCGAGATTTCGTTCTTCTCTTCAAGG-3'

Other clones with same target sequence:

  1. TRCN0000074170