Construct: shRNA TRCN0000333552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001000.2-230s21c1
DNA Barcode:
GCACATGAGATGGCACACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL39 (6170)
Gene Description:
ribosomal protein L39
Transcript:
RefSeq NM_001000.2 (NON-CURRENT)
Match location:
Position 230 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATGAGATGGCACACATA
Hairpin Sequence:
5'-CCGG-GCACATGAGATGGCACACATA-CTCGAG-TATGTGTGCCATCTCATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATGAGATGGCACACATACTCGAGTATGTGTGCCATCTCATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATGAGATGGCACACATACTCGAGTATGTGTGCCATCTCATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000117632