Construct: shRNA TRCN0000333563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000387.4-1016s21c1
DNA Barcode:
GCGGCCTGTTTCCTTGGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A20 (788)
Gene Description:
solute carrier family 25 member 20
Transcript:
RefSeq NM_000387.4 (NON-CURRENT)
Match location:
Position 1016 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCCTGTTTCCTTGGCTTT
Hairpin Sequence:
5'-CCGG-GCGGCCTGTTTCCTTGGCTTT-CTCGAG-AAAGCCAAGGAAACAGGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCCTGTTTCCTTGGCTTTCTCGAGAAAGCCAAGGAAACAGGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCCTGTTTCCTTGGCTTTCTCGAGAAAGCCAAGGAAACAGGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000044547