Construct: shRNA TRCN0000333583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014216.4-790s21c1
DNA Barcode:
CGAGATGGCTATCGTGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPK1 (3705)
Gene Description:
inositol-tetrakisphosphate 1-kinase
Transcript:
RefSeq NM_014216.4 (NON-CURRENT)
Match location:
Position 790 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGGCTATCGTGTTCAA
Hairpin Sequence:
5'-CCGG-CGAGATGGCTATCGTGTTCAA-CTCGAG-TTGAACACGATAGCCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGGCTATCGTGTTCAACTCGAGTTGAACACGATAGCCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGGCTATCGTGTTCAACTCGAGTTGAACACGATAGCCATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000037695