Construct: shRNA TRCN0000333585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001822.4-962s21c1
DNA Barcode:
GCACGTAGGATACACAACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHN1 (1123)
Gene Description:
chimerin 1
Transcript:
RefSeq NM_001822.4 (NON-CURRENT)
Match location:
Position 962 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGTAGGATACACAACCTT
Hairpin Sequence:
5'-CCGG-GCACGTAGGATACACAACCTT-CTCGAG-AAGGTTGTGTATCCTACGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGTAGGATACACAACCTTCTCGAGAAGGTTGTGTATCCTACGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGTAGGATACACAACCTTCTCGAGAAGGTTGTGTATCCTACGTGC-3'

Other clones with same target sequence:

  1. TRCN0000047799