Construct: shRNA TRCN0000333602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001022.3-504s21c1
DNA Barcode:
GCTTGCTCCCTACGATGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS19 (6223)
Gene Description:
ribosomal protein S19
Transcript:
RefSeq NM_001022.3 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCTCCCTACGATGAGAA
Hairpin Sequence:
5'-CCGG-GCTTGCTCCCTACGATGAGAA-CTCGAG-TTCTCATCGTAGGGAGCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCTCCCTACGATGAGAACTCGAGTTCTCATCGTAGGGAGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCTCCCTACGATGAGAACTCGAGTTCTCATCGTAGGGAGCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000074916