Construct: shRNA TRCN0000333715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181581.1-1241s21c1
DNA Barcode:
GCACATCAGCAATCATAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUS4L (11062)
Gene Description:
dihydrouridine synthase 4 like
Transcript:
RefSeq NM_181581.1 (NON-CURRENT)
Match location:
Position 1241 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATCAGCAATCATAGATT
Hairpin Sequence:
5'-CCGG-GCACATCAGCAATCATAGATT-CTCGAG-AATCTATGATTGCTGATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATCAGCAATCATAGATTCTCGAGAATCTATGATTGCTGATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATCAGCAATCATAGATTCTCGAGAATCTATGATTGCTGATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000064855