Construct: shRNA TRCN0000333827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015135.2-2486s21c1
DNA Barcode:
GCTCTCAGTTTACTGGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUP205 (23165)
Gene Description:
nucleoporin 205
Transcript:
RefSeq NM_015135.2 (NON-CURRENT)
Match location:
Position 2486 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCAGTTTACTGGAAGAA
Hairpin Sequence:
5'-CCGG-GCTCTCAGTTTACTGGAAGAA-CTCGAG-TTCTTCCAGTAAACTGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCAGTTTACTGGAAGAACTCGAGTTCTTCCAGTAAACTGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCAGTTTACTGGAAGAACTCGAGTTCTTCCAGTAAACTGAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000060024