Construct: shRNA TRCN0000333919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016768.1-778s21c1
DNA Barcode:
CGTGTGAAGCAGTTATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbx3 (18516)
Gene Description:
pre B cell leukemia homeobox 3
Transcript:
RefSeq NM_016768.1 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTGAAGCAGTTATGATTT
Hairpin Sequence:
5'-CCGG-CGTGTGAAGCAGTTATGATTT-CTCGAG-AAATCATAACTGCTTCACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTGAAGCAGTTATGATTTCTCGAGAAATCATAACTGCTTCACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTGAAGCAGTTATGATTTCTCGAGAAATCATAACTGCTTCACACG-3'

Other clones with same target sequence:

  1. TRCN0000075385