Construct: shRNA TRCN0000333931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198052.1-1737s21c1
DNA Barcode:
GCGAATGTTCCCTCCGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbx3 (21386)
Gene Description:
T-box 3
Transcript:
RefSeq NM_198052.1 (NON-CURRENT)
Match location:
Position 1737 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAATGTTCCCTCCGTTTAA
Hairpin Sequence:
5'-CCGG-GCGAATGTTCCCTCCGTTTAA-CTCGAG-TTAAACGGAGGGAACATTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAATGTTCCCTCCGTTTAACTCGAGTTAAACGGAGGGAACATTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAATGTTCCCTCCGTTTAACTCGAGTTAAACGGAGGGAACATTCGC-3'

Other clones with same target sequence:

  1. TRCN0000095870