Construct: shRNA TRCN0000333985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033713.1-839s21c1
DNA Barcode:
CCAGCTCAACATTAGCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mef2a (17258)
Gene Description:
myocyte enhancer factor 2A
Transcript:
RefSeq NM_001033713.1 (NON-CURRENT)
Match location:
Position 839 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTCAACATTAGCAGATT
Hairpin Sequence:
5'-CCGG-CCAGCTCAACATTAGCAGATT-CTCGAG-AATCTGCTAATGTTGAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTCAACATTAGCAGATTCTCGAGAATCTGCTAATGTTGAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTCAACATTAGCAGATTCTCGAGAATCTGCTAATGTTGAGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000095960