Construct: shRNA TRCN0000334274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019413.2-3481s21c1
DNA Barcode:
CCAGTTAGATTCTCACGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Robo1 (19876)
Gene Description:
roundabout guidance receptor 1
Transcript:
RefSeq NM_019413.2 (CURRENT)
Match location:
Position 3481 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTAGATTCTCACGGAAA
Hairpin Sequence:
5'-CCGG-CCAGTTAGATTCTCACGGAAA-CTCGAG-TTTCCGTGAGAATCTAACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTAGATTCTCACGGAAACTCGAGTTTCCGTGAGAATCTAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTAGATTCTCACGGAAACTCGAGTTTCCGTGAGAATCTAACTGG-3'

Other clones with same target sequence:

  1. TRCN0000100069