Construct: shRNA TRCN0000334407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011048.1-1868s21c1
DNA Barcode:
GCTGAAAGAATGGAGCCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcsk6 (18553)
Gene Description:
proprotein convertase subtilisin/kexin type 6
Transcript:
RefSeq NM_011048.1 (CURRENT)
Match location:
Position 1868 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAAGAATGGAGCCTCAT
Hairpin Sequence:
5'-CCGG-GCTGAAAGAATGGAGCCTCAT-CTCGAG-ATGAGGCTCCATTCTTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAAGAATGGAGCCTCATCTCGAGATGAGGCTCCATTCTTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAAGAATGGAGCCTCATCTCGAGATGAGGCTCCATTCTTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000221979