Construct: shRNA TRCN0000334559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025471.2-436s21c1
DNA Barcode:
GCTCTTCATTAAACCCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smim8 (66291)
Gene Description:
small integral membrane protein 8
Transcript:
RefSeq NM_025471.2 (NON-CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTCATTAAACCCAACAA
Hairpin Sequence:
5'-CCGG-GCTCTTCATTAAACCCAACAA-CTCGAG-TTGTTGGGTTTAATGAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTCATTAAACCCAACAACTCGAGTTGTTGGGTTTAATGAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTCATTAAACCCAACAACTCGAGTTGTTGGGTTTAATGAAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000127322