Construct: shRNA TRCN0000334575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146133.2-615s21c1
DNA Barcode:
GAGACATGGAACCCTTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golph3l (229593)
Gene Description:
golgi phosphoprotein 3-like
Transcript:
RefSeq NM_146133.2 (NON-CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACATGGAACCCTTTCAAA
Hairpin Sequence:
5'-CCGG-GAGACATGGAACCCTTTCAAA-CTCGAG-TTTGAAAGGGTTCCATGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACATGGAACCCTTTCAAACTCGAGTTTGAAAGGGTTCCATGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACATGGAACCCTTTCAAACTCGAGTTTGAAAGGGTTCCATGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000111669