Construct: shRNA TRCN0000334597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007791.5-577s21c2
DNA Barcode:
CTGTAAAGGATGCTATGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csrp1 (13007)
Gene Description:
cysteine and glycine-rich protein 1
Transcript:
RefSeq NM_007791.5 (CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTAAAGGATGCTATGCCAA
Hairpin Sequence:
5'-CCGG-CTGTAAAGGATGCTATGCCAA-CTCGAG-TTGGCATAGCATCCTTTACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTAAAGGATGCTATGCCAACTCGAGTTGGCATAGCATCCTTTACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTAAAGGATGCTATGCCAACTCGAGTTGGCATAGCATCCTTTACAG-3'

Other clones with same target sequence:

  1. TRCN0000125082