Construct: shRNA TRCN0000334608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019837.2-497s21c3
DNA Barcode:
TGGGAAGACTAGTTGGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt3 (56409)
Gene Description:
nudix (nucleotide diphosphate linked moiety X)-type motif 3
Transcript:
RefSeq NM_019837.2 (CURRENT)
Match location:
Position 497 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAAGACTAGTTGGAATTT
Hairpin Sequence:
5'-CCGG-TGGGAAGACTAGTTGGAATTT-CTCGAG-AAATTCCAACTAGTCTTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAAGACTAGTTGGAATTTCTCGAGAAATTCCAACTAGTCTTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAAGACTAGTTGGAATTTCTCGAGAAATTCCAACTAGTCTTCCCA-3'

Other clones with same target sequence:

  1. TRCN0000080626