Construct: shRNA TRCN0000334640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025939.2-1515s21c1
DNA Barcode:
CCAGTGGTATTGGCTGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Paics (67054)
Gene Description:
phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase
Transcript:
RefSeq NM_025939.2 (NON-CURRENT)
Match location:
Position 1515 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGGTATTGGCTGTTCAA
Hairpin Sequence:
5'-CCGG-CCAGTGGTATTGGCTGTTCAA-CTCGAG-TTGAACAGCCAATACCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGGTATTGGCTGTTCAACTCGAGTTGAACAGCCAATACCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGGTATTGGCTGTTCAACTCGAGTTGAACAGCCAATACCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000076100