Construct: shRNA TRCN0000334836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146141.1-516s21c1
DNA Barcode:
CGTCTGTGAAATAGGCTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppa2 (74776)
Gene Description:
pyrophosphatase (inorganic) 2
Transcript:
RefSeq NM_146141.1 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGTGAAATAGGCTCAAA
Hairpin Sequence:
5'-CCGG-CGTCTGTGAAATAGGCTCAAA-CTCGAG-TTTGAGCCTATTTCACAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGTGAAATAGGCTCAAACTCGAGTTTGAGCCTATTTCACAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGTGAAATAGGCTCAAACTCGAGTTTGAGCCTATTTCACAGACG-3'

Other clones with same target sequence:

  1. TRCN0000114161