Construct: shRNA TRCN0000334939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019824.3-167s21c1
DNA Barcode:
GAGACCAAAGACACGGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arpc3 (56378)
Gene Description:
actin related protein 2/3 complex, subunit 3
Transcript:
RefSeq NM_019824.3 (NON-CURRENT)
Match location:
Position 167 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCAAAGACACGGACATT
Hairpin Sequence:
5'-CCGG-GAGACCAAAGACACGGACATT-CTCGAG-AATGTCCGTGTCTTTGGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCAAAGACACGGACATTCTCGAGAATGTCCGTGTCTTTGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCAAAGACACGGACATTCTCGAGAATGTCCGTGTCTTTGGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000110782