Construct: shRNA TRCN0000334976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025295.3-333s21c1
DNA Barcode:
GCAGATTATAGTGTTCCCAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btd (26363)
Gene Description:
biotinidase
Transcript:
RefSeq NM_025295.3 (NON-CURRENT)
Match location:
Position 333 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTATAGTGTTCCCAGA
Hairpin Sequence:
5'-CCGG-GCAGATTATAGTGTTCCCAGA-CTCGAG-TCTGGGAACACTATAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTATAGTGTTCCCAGACTCGAGTCTGGGAACACTATAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTATAGTGTTCCCAGACTCGAGTCTGGGAACACTATAATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000101409