Construct: shRNA TRCN0000335049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175025.3-910s21c1
DNA Barcode:
CCTGCGGACTTACGCTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2c1 (235574)
Gene Description:
ATPase, Ca++-sequestering
Transcript:
RefSeq NM_175025.3 (NON-CURRENT)
Match location:
Position 910 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCGGACTTACGCTTATTT
Hairpin Sequence:
5'-CCGG-CCTGCGGACTTACGCTTATTT-CTCGAG-AAATAAGCGTAAGTCCGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCGGACTTACGCTTATTTCTCGAGAAATAAGCGTAAGTCCGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCGGACTTACGCTTATTTCTCGAGAAATAAGCGTAAGTCCGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000101426