Construct: shRNA TRCN0000335117
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030113.2-1594s21c1
- DNA Barcode:
- CCGAGTTAATGCCATCCACTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Arhgap10 (78514)
- Gene Description:
- Rho GTPase activating protein 10
- Transcript:
- RefSeq NM_030113.2 (CURRENT)
- Match location:
- Position 1594 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000335117)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGAGTTAATGCCATCCACTT
- Hairpin Sequence:
-
5'-CCGG-CCGAGTTAATGCCATCCACTT-CTCGAG-AAGTGGATGGCATTAACTCGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGAGTTAATGCCATCCACTTCTCGAGAAGTGGATGGCATTAACTCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGAGTTAATGCCATCCACTTCTCGAGAAGTGGATGGCATTAACTCGG-3'
Other clones with same target sequence:
-
TRCN0000094796
