Construct: shRNA TRCN0000335178

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133772.1-885s21c1
DNA Barcode:
CGAGAATATGTACACCATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssbp4 (76900)
Gene Description:
single stranded DNA binding protein 4
Transcript:
RefSeq NM_133772.1 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335178)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAATATGTACACCATCAT
Hairpin Sequence:
5'-CCGG-CGAGAATATGTACACCATCAT-CTCGAG-ATGATGGTGTACATATTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAATATGTACACCATCATCTCGAGATGATGGTGTACATATTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAATATGTACACCATCATCTCGAGATGATGGTGTACATATTCTCG-3'

Other clones with same target sequence:

  1. TRCN0000108594