Construct: shRNA TRCN0000335196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030113.2-1958s21c1
DNA Barcode:
GCCGTGTACAACCTTTGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap10 (78514)
Gene Description:
Rho GTPase activating protein 10
Transcript:
RefSeq NM_030113.2 (CURRENT)
Match location:
Position 1958 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGTACAACCTTTGTCTT
Hairpin Sequence:
5'-CCGG-GCCGTGTACAACCTTTGTCTT-CTCGAG-AAGACAAAGGTTGTACACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGTACAACCTTTGTCTTCTCGAGAAGACAAAGGTTGTACACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGTACAACCTTTGTCTTCTCGAGAAGACAAAGGTTGTACACGGC-3'

Other clones with same target sequence:

  1. TRCN0000094797