Construct: shRNA TRCN0000335227
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_199448.2-590s21c1
- DNA Barcode:
- CCAGGAGATTCAGACTCTCAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Fez2 (225020)
- Gene Description:
- fasciculation and elongation protein zeta 2 (zygin II)
- Transcript:
- RefSeq NM_199448.2 (NON-CURRENT)
- Match location:
- Position 590 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000335227)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGGAGATTCAGACTCTCAA
- Hairpin Sequence:
-
5'-CCGG-CCAGGAGATTCAGACTCTCAA-CTCGAG-TTGAGAGTCTGAATCTCCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGGAGATTCAGACTCTCAACTCGAGTTGAGAGTCTGAATCTCCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGGAGATTCAGACTCTCAACTCGAGTTGAGAGTCTGAATCTCCTGG-3'
Other clones with same target sequence:
-
TRCN0000054635
