Construct: shRNA TRCN0000335291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199448.2-332s21c1
DNA Barcode:
CCATACAAGGACCTTGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fez2 (225020)
Gene Description:
fasciculation and elongation protein zeta 2 (zygin II)
Transcript:
RefSeq NM_199448.2 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACAAGGACCTTGCATTT
Hairpin Sequence:
5'-CCGG-CCATACAAGGACCTTGCATTT-CTCGAG-AAATGCAAGGTCCTTGTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACAAGGACCTTGCATTTCTCGAGAAATGCAAGGTCCTTGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACAAGGACCTTGCATTTCTCGAGAAATGCAAGGTCCTTGTATGG-3'

Other clones with same target sequence:

  1. TRCN0000054633