Construct: shRNA TRCN0000335359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025909.3-705s21c1
DNA Barcode:
CGGAAGGAGTAAACTACTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oma1 (67013)
Gene Description:
OMA1 zinc metallopeptidase
Transcript:
RefSeq NM_025909.3 (CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGGAGTAAACTACTGTT
Hairpin Sequence:
5'-CCGG-CGGAAGGAGTAAACTACTGTT-CTCGAG-AACAGTAGTTTACTCCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGGAGTAAACTACTGTTCTCGAGAACAGTAGTTTACTCCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGGAGTAAACTACTGTTCTCGAGAACAGTAGTTTACTCCTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000031082