Construct: shRNA TRCN0000335501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011721.4-3595s21c1
DNA Barcode:
GCAAGGCAGAAACACGCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wrn (22427)
Gene Description:
Werner syndrome RecQ like helicase
Transcript:
RefSeq NM_011721.4 (CURRENT)
Match location:
Position 3595 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGCAGAAACACGCTAAT
Hairpin Sequence:
5'-CCGG-GCAAGGCAGAAACACGCTAAT-CTCGAG-ATTAGCGTGTTTCTGCCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGCAGAAACACGCTAATCTCGAGATTAGCGTGTTTCTGCCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGCAGAAACACGCTAATCTCGAGATTAGCGTGTTTCTGCCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000071016