Construct: shRNA TRCN0000335748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007616.3-560s21c1
DNA Barcode:
TGAAGCTATTGGCAAGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cav1 (12389)
Gene Description:
caveolin 1, caveolae protein
Transcript:
RefSeq NM_007616.3 (NON-CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGCTATTGGCAAGATATT
Hairpin Sequence:
5'-CCGG-TGAAGCTATTGGCAAGATATT-CTCGAG-AATATCTTGCCAATAGCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGCTATTGGCAAGATATTCTCGAGAATATCTTGCCAATAGCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGCTATTGGCAAGATATTCTCGAGAATATCTTGCCAATAGCTTCA-3'

Other clones with same target sequence:

  1. TRCN0000112663